diff --git a/yt_dlp/extractor/twitch.py b/yt_dlp/extractor/twitch.py
index f0a9a24bd9..2de5552c4f 100644
--- a/yt_dlp/extractor/twitch.py
+++ b/yt_dlp/extractor/twitch.py
@@ -204,9 +204,9 @@ class TwitchBaseIE(InfoExtractor):
         for fmt in formats:
             if fmt.get('vcodec') and fmt['vcodec'].startswith('av01'):
                 # mpegts does not yet have proper support for av1
-                fmt['downloader_options'] = {'ffmpeg_args_out': ['-f', 'mp4']}
+                fmt.setdefault('downloader_options', {})['ffmpeg_args_out'] = ['-f', 'mp4']
             if self.get_param('live_from_start'):
-                fmt['downloader_options'] = {'ffmpeg_args': ['-live_start_index', '0']}
+                fmt.setdefault('downloader_options', {})['ffmpeg_args'] = ['-live_start_index', '0']
 
         return formats